In this study, we developed a novel, multiplex qPCR assay for simultaneous detection of RIG-1, MDA5, and Concealers IFIT-1 at the mRNA level.The assay was validated in A549 cells transfected with in vitro transcribed RNAs.Both exogenous RNA-GFP and self-amplifying (saRNA-GFP) induced significant expression of RIG-1, MDA5, IFIT-1, as well as type I
Research on slope stability of Fushun West Open-pit Mine based on global critical slip field technology
Based on the principle of global critical slip field, this paper applies four evaluation methods of Ordinary, Bishop, Janbu and Morgenstern-price to establish a global and refined evaluation system for slope stability.The evaluation system was applied to the comprehensive evaluation of the slope stability before and after the presser foot discharge